Abstract
The model of biological cell conductivity as equivalent nonlinear electric scheme is represented. A model assumes that structural elements of cell are a membrane and cytoplasm, can be represented as a resistor-capacity circuit, the calculation of which can be executed by known rules for electric elements and connections. Equation of nonlinear function of single cell conductivity is got, and on this basis and conductivity of 2-cell embryo, depending on the external voltage put by means of microelectrodes on the method of pulse conductometry. It is shown that for 2-cell embryo additionally there is dependence of conductivity on cell orientation in relation to the microelectrodes axis. The built electric model adequately explains the experiments on measuring of cells conductivity in pulse electric field. References 9, figures 5.
References
Antonov V.F., Chernysch A.M., Pasechnik V.I., Voznesenskii S.A., Kozlova E.K. Biophysics: Textbook for students of higher schools. – Moskva: VLADOS, 2000. – 293 p. (Rus)
Bluhm H. Pulsed power systems. Principles and applications, Berlin:Springer-Verlag. – 2006. – 326 p.
Gimsa J., Wachner D. A unified resistor-capacitor model for impedance, dielectrophoresis, electrorotation, and induced transmembrane potential // Biophysical Journal. – 1998. – Vol.75. – Pp. 1107–1116.
Zernov N.V., Karpov V.G. Theory of radio engineering circuits. – Leningrad: Energiia, 1972. – 816 p. (Rus)
Krassowska W., Filev P.D. Modeling Electroporation in and Single Cell // Biophysical Journal. – 2007. – Vol. 92. – Pp. 404–417.
Pakhomov A.G., Miklavcic D., Markov M.S. Advanced Electroporation Techniques in Biology and Medicine. – New York: CRC Press, 2010. – 528 р.
Schwan H.P. Electrical properties of tissue and cell suspensions / In Advances in Biological and Medical Physics, N.Y:Academy Press, 1957. – Рp. 147–209.
Shigimaga V.A., Megel Ju.E. Application of pulse conductometry method for research of electric characteristics of biological cells // Pratsi Instytutu elektrodynamiky Natsionalnoi Akademii Nauk Ukrainy. – 2012. – Vypusk 31. – Pp. 147–155. (Rus)
Shigimaga V.A. Pulse conductometer for biological cells and liquid media // Izmeritelnaia Tekhnika. – 2012. – №11. – Pp. 45–49. (Rus)

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